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sucD [2019-08-01 11:16:40]
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sucD [2019-08-01 11:16:40]

succinyl-CoA synthetase (alpha subunit)
locus
BSU_16100
pI
5.59
mw
31.23 kDa
protein length
300 aa Sequence Blast
gene length
903 bp Sequence Blast
function
TCA cycle
product
succinyl-CoA synthetase (alpha subunit)
essential
no
ec
6.2.1.5
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.5|ATP synthesis] → [category|SW 2.1.5.2|Substrate-level phosphorylation]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • Gene

    Coordinates
    1,681,617 1,682,519

    The protein

    Catalyzed reaction/ biological activity

  • ATP succinate CoA = ADP phosphate succinyl-CoA (according to Swiss-Prot)

    • Protein family

  • succinate/malate CoA ligase alpha subunit family (single member, according to UniProt)

    • Kinetic information

  • Reversible Michaelis-Menten [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]

    • Modification

  • phosphorylation on (Ser-19 OR Thr-20) [Pubmed|17218307]

    • Effectors of protein activity

  • Inhibited by 2-oxoglutarate, ATP and NADH [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
  • GTP is not accepted by the enzyme [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]

    • Structure

  • [PDB|1JKJ] (E. coli)

    • Additional information

  • extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at [http://www.proteopedia.org/wiki/index.php/Succinyl-Coa_Synthetase Proteopedia]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|12850135]
  • expression is heterogeneous [pubmed|29809139]
    • view in new tab

    Biological materials

    Mutant

  • GP720 (spc), available in [SW|Jörg Stülke]'s lab
  • 1A1008 ( ''sucD''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1008&Search=1A1008 BGSC]
  • GP791 ∆(''[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]'')::''tet'', available in [SW|Jörg Stülke]'s lab
  • GP2344 ∆(''[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]'')::''kan'', Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab
  • GP2345 ∆(''[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]''::''lox72''), Cre-recombinase is integrated in ''[gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA]'', available in [SW|Jörg Stülke]'s lab
  • BKE16100 ∆([gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD])::erm trpC2 available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTGGTCCCCTGCCT, downstream forward: _UP4_TAATAAAAAAGGGACAGCCG
  • BKK16100 ∆([gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD])::kan trpC2 available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTTGGTCCCCTGCCT, downstream forward: _UP4_TAATAAAAAAGGGACAGCCG

    • GFP fusion

  • GP1435 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1425 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • **

    • References

  • 12850135,17218307,11976317,20933603,15378759,29809139